Journal: Nutrients

Article Title: Immunomodulatory Effects of Lactobacillus brevis NES-428 in a Hyperthyroidism Mouse Model: Potential Applications for Graves’ Disease

doi: 10.3390/nu17182967

Figure Lengend Snippet: NES-428 modulates pro- and anti-inflammatory cytokine transcription in Jurkat T cells. In the non-treated (NC) group, the cells were simply maintained for 24 h without any stimulation. In the stimulated control (ST) group, the same number of cells was exposed to 50 ng/mL of phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 h. For the NES-428 group, Jurkat cells were first incubated for 24 h with heat-killed NES-428, after which they were stimulated by 50 ng/mL PMA and 1 µg/mL ionomycin for 6 h. Total RNA was extracted from each group, and cytokine expression was analyzed by quantitative real-time PCR. Relative mRNA expression of IL-2, IL-4, IL-6, IL-12, TNF-α and IFN-γ was shown. NC = untreated control; ST = stimulated positive control. Data represent mean ± SD of three independent experiments; p < 0.05 vs. NC.

Article Snippet: To investigate the immunomodulatory effects of NES-428, the human T lymphocyte cell line Jurkat (ATCC TIB-152) was employed for cytokine expression analysis.

Techniques: Control, Incubation, Expressing, Real-time Polymerase Chain Reaction, Positive Control

Journal: Journal of Clinical Medicine

Article Title: Validation of a Ready-to-Use Lyophilized Kit for Labeling IL2 with 68 Ga: A New Avenue for Imaging Activated T-lymphocytes in Tumor Microenvironment

doi: 10.3390/jcm14165658

Figure Lengend Snippet: Flow cytometric analysis of Treg purity and CD25 expression. The cytograms show, in the upper line, CD4 vs. dump channel markers (dead cell dye, CD14/CD16/CD19/CD8), and, in the lower line, CD25 vs. FOXP3 expression in gated CD4 T-cells. Right, histogram overlay of CD25 expression in the indicated cell subsets; numbers shown the geometric mean fluorescence intensity.

Article Snippet: - CD8+ T-lymphocytes were isolated using the CD8+ T Cell Isolation Kit, human 130-096-495 for 10 9 total cells (Miltenyi Biotec).

Techniques: Expressing, Fluorescence